POTENTIAL ANTI-VIRAL ACTIVITIES (%) OF THREE ORGANIC SOLVENT FRACTIONS OF WILD GANODERMA LUCIDUM (HIGHER BASIDIOMYCETE) EXTRACT AGAINST INFECTIOUS BURSAL DISEASE (IBD)
B. Ramya Sree
Department of Biotechnology, Sree Vidyanikethan Engineering College, Tirupati
Jyothi M. Joy
Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati
The anti-viral activity of different organic fractions of Ganoderma lucidum extract against infectious bursal disease (IBD) virus was calculated by relative optical density absorbance method. Fractions of methanol, ethyl acetate and n-butanol of the mushroom were tested against non neuraminidase producing IBD virus as antigen using fetuin as substrate. The antigen was serially diluted to 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256, 1:512 and 1:1024 (converted to Log10- 3.2, 1.8, 1.3, 1.2, 1.1, 1.0, 1.0,1.0, 1.0, 1.0) and used the optical density absorbance was measured using spectrometer. Calculated viral inhibitory activity of the methanolic soluble fraction of Ganoderma lucidum extract was 1.6 %, 3.5 %, 1.8 % and 1.4 % at viral concentrations (log10) of 1.3, 1.0, 1.0 and 1.0 respectively. Ethyl acetate soluble fraction of the extract showed inhibitory activity of 2.9 %, 2.3 %, 16.6 % and 17.6 % at viral concentration (log10) of 1.3, 1.0, 1.0 and 1.0. The n-butanol soluble fraction of the extract showed inhibitory activity against IBD virus antigen of 15.5 %, 8.5 %, 12.7 % and 3.9 % and 11.1 % at viral concentrations (log10) of 3.2, 1.8, 1.0, 1.0 and 1.0 respectively. The finding indicates that n-butanol fraction of Ganoderma lucidum extract inhibits infectious bursal disease viral growth compared to methanol and ethyl acetate fractions and can be exploited in the management of infectious bursal disease infection in poultry.
4 , 3 , 2014
133 - 137