ANTIBACTERIAL ACTIVITY OF TAGETES MINUTA L. AGAINST STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS PYOGENES
B. Ramya Sree
Department of Biotechnology, Sree Vidyanikethan Engineering College, Tirupati
Jyothi M. Joy
Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati
Evaluated the antibacterial effect of aqueous and ethanolic extract of the leaf, bark of Tagetes minuta L. against Staphylococcus aureus and Streptococcus pyogenes was analyzed in this study. These Gram positive strains were isolated and characterized by standard methods. Aqueous and ethanolic extract of the leaf, bark of Tagetes minuta L. were prepared with help of soxhlet unit. Further, evaluated the antimicrobial activity of these extract were analyzed against S. aureus and S. pyogenes. Biochemical test confirmed that isolated strains were Staphylococcus aureus and Streptococcus pyogenes. Aqueous and ethanolic extract of the leaf and bark of Tagetes minuta leaf showed significant antibacterial activity against Staphylococcus aureus and Streptococcus pyogens. 15 μg/ml aqueous extract of T. minuta leaf showed 80, 120% antimicrobial activity against Staphylococcus aureus and Streptococcus pyogens respectively as compared to the control. Similarly, ethanolic extract of leaf of T.s minuta showed significant values against S. aureus and S. pyogens. 15 ethanolic extracts of T. minuta leaf showed more inhibition zones (121.21 and 0 %, respectively) against Staphylococcus aureus and Streptococcus pyogens. 15 μg/ml aqueous extract of bark of T. minuta showed less inhibition zone against Staphylococcus aureus as compared to 15 μg/ml norflox (control drug) whereas 35 μg/ml aqueous extract of bark of T. minuta exhibited more inhibition zone (49.59%) against S. pyogens as compared to the control. Similarly, 20 μg/ml ethanolic extract T. minuta bark marked similar inhibition zone against S. aureus and similarly 20 μg/ml ethanolic extract T. minuta bark showed 50.31% more inhibition zone against S. pyogens as compared to 15 μg/ml norflox (control drug). Aqueous extract of the leaves as well as barks of Tagetes minuta exhibited 5 μg/ml MIC against S. aureus and S. pyogenes.
4 , 2 , 2014
109 - 118