THE EFFECT OF GROWTH REGULATORS ON SHOOT INITIATION, MULTIPLICATION AND ROOTING OF LAVENDER (Lavandula angustifolia L.) - AN SIGNIFICANT MEDICINAL PLANT

B. Ramya Sree
Department of Biotechnology, Sree Vidyanikethan Engineering College, Tirupati
Jyothi M. Joy
Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati

Autoimmune diseases ,autoantibodies ,Antimicrobial Peptides ,

Lavandula angustifolia-Important Medicinal Plant, Nodal segment from micro propagated plants were used to evaluate the effect of growth regulators on the in vitro callusing and shoot proliferation and rooting from apical bud explants supplemented with BAP (8.88 μΜ ) and NAA (5.36 μM) . Rooting of the plantlets were obtained, when these shoots were inoculated on rooting medium, MSBM fortified with BAP (8.88μΜ) + NAA (2.68μΜ) + IBA (4.92μΜ) after 38 days of culture. However, rooting rate and root growth increased with increased concentration of NAA with half strength of MS medium. The plantlets were successfully transferred to soil and were grown till they reached maturity. MS basal medium supplemented with BAP 4.44 μΜ + Kn 4.64 μΜ + NAA 2.68 μΜ produced highest frequency of shoots regeneration with highest number of long shoots. This established normal growth and development with 75-85% survival frequency.

4 , 2 , 2014