Water deficit is an environmental factor that may influence grain yield and nutrient uptake of wheat (Triticum aestivum L.). The objective of this study was to evaluate the effects of water deficit on grain yield micronutrient uptake of bread wheat cultivars. Two field experiments were conducted at the Agriculture Research Station of Saatlo in West Azarbayjan during 2010-11 and 2011-12 to examine the effects of water supply (normal irrigation and post anthesis drought stress condition) on micronutrients uptakes and grain yield in five winter wheat cultivars (Zarrin, Peshgam, Orum, Zareh and Mihan). Irrigation and cultivars showed significant differences in grain yield, and micronutrients uptakes. Grain yield and grain micronutrients uptakes (Fe, Zn, Mn and Cu) in all cultivars decreased with decreasing water availability. These results suggest that wheat cultivars respond differentially to water deficit and macronutrients. The highest grain yield per unit area was obtained under well-irrigation and water deficit in Zareh and Mihan cultivars, respectively. The highest Zn uptake by grains under well-irrigation was observed in Peshgam cultivar. Zareh cultivar had the most Fe, Mn and Cu uptake under well-irrigation. However under water deficit condition Mihan has the highest Fe, Zn, Mn and Cu uptake by grains.