Acrylamide is a vinyl monomer with established neurotoxic effects. The important source for human exposure to acrylamide is occupational and other exposure sources include drinking water, smoking and foods cooked at high temperatures. In present study we evaluated the effects of acrylamide on neurobehavioral and locomotor functions under standard, validated test battery in wistar rats. Acrylamide administration at dose level of 40 mg/kg body weight for 14 days in both male and female rats induced significant characteristic neurotoxic symptoms including gait abnormalities, increased foot splay, decrease in grip strength and decrease in locomotor activity.