Many Siddha medicines have been used in the treatment of urinary calculi. But a lacuna of inadequate scientific evaluation in understanding the mode of action exists till now. To fulfill this lacuna, in the present study an effort has been made to evaluate the Antiurolithiatic activity of Tinospora sinensis in ethylene glycol induced lithiasis in rats. Experimentally induced urolithiatic rats were treated with standard drug cystone and trial drug Tinospora sinensis in two different doses (1ml and 2ml/kg). The calcium, phosphate and oxalate content in urine and kidney homogenate were determined and serum estimation of creatinine, uric acid and urea nitrogen were performed on 28th day of experiment and were compared to the normal, control and standard drug group (cystone). The results suggested that Tinospora sinensis at the dose of 2ml/kg provided lowering effect of urinary stone forming constituents which was closer to standard group on dose dependent manner. So the present work was confirmed the traditional use of Tinospora sinensis for antiurolithiatic activity