The involvement of pharmacists and pharmacy students in public health is increasing, so pharmacists and pharmacy students have an opportunity to participate. well-being, good health, preventing diseases education is provided to the community. Likewise, this study aims to examine how a health promotion program affects college women's knowledge and use of multivitamins on the knowledge and use of multivitamins in general and on neural tube defects (NTDs) in particular. Pharmacy students and two pharmacy faculty members developed a health promotion program including a presentation and reminders based on consultations with two pharmacy faculty members. An assessment of participants' knowledge of folic acid and NTDs was given before and immediately after the presentation. Folic acid was reminded to participants every week for three weeks. The knowledge and use of multivitamins were assessed after four weeks. Sixteen college women volunteered to presentations will be held orally. The four-week posttest was completed by twelve females (78.2%). As well as correct answers regarding Vitamin B9 and NTD, a statistically significant increase in test scores was observed after the test (p = 0.0001). Multivitamin use among participants increased statistically significantly (p=0.023). The pilot health promotion program significantly improved participants' knowledge of vitamin B9 and multivitamins. Increasing college women's knowledge of folic acid and NTD could be promoted through a similar health promotion program