To evaluate the pharmacological potential and to perform the phytochemical characterization of the dry hydroethanolic extract of the leaves of Raphanus sativus (nabo forrageiro). The leaves of this species are used worldwide because of its medicinal properties. Material and Methods: In vivo anti-inflammatory and antinociceptive effects of dry hydroethanolic extract of the leaves were evaluated through lipopolysaccharide (LPS)-induced peritonitis and carrageenan-induced paw edema tests and by the formalin test, respectively. In this study we used adult male Swiss mice. To elucidate the compounds present in the extract, a phytochemical analysis was performed using high performance liquid chromatography (HPLC). Results and Conclusions: The extract induced anti-inflammatory activity as determined by the LPS-induced peritonitis and carrageenan-induced paw edema tests. The extract also showed activity in the formalin test. Additionally, on the basis of HPLC data, the structures of three flavonoids, kaempferol, rutin, and quercetin, which can be associated with the biological activity of R. sativus, were identified.