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Issn  2249-7579
e Issn  2249-7579
Publisher JOURNAL OF PHARMACEUTICAL BIOLOGY

SPECTROPHOTOMETRIC DETERMINATION OF MEROPENEM IN BULK AND INJECTION FORMULATIONS BY BRUCINE

B. Ramya Sree
Department of Biotechnology, Sree Vidyanikethan Engineering College, Tirupati
Jyothi M. Joy
Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati

Autoimmune diseases ,autoantibodies ,Antimicrobial Peptides ,

A simple and cost effective spectrophotometric method was described for the determination of Meropenem in pure form and in pharmaceutical formulations. The method is based on the formation of colored chromogen when the drug reacts with brucine and sodium periodate in acidic medium. This method was applied for the determination of drug contents in pharmaceutical formulations and enabled the determination of the selected drug in microgram quantities (0.5 to 3.0 mL). No interferences were observed from excipients and the validity of the method was tested against reference method. The colored species has an absorption maximum at 523 nm for Meropenem and obeys beer’s law in the concentration range 2-12 μg/mL of Meropenem. The apparent molar absorptivity was 11x10-5 and sandell’s sensitivity was 7x10-2. The slope is 0.0310 ± 0.0004, the intercept of the equation of the regression line is -0.0006 ± 0.0006. The optimum experimental parameters for the reaction have been studied and the validity of the described procedure was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied for the determination of Meropenem in pharmaceutical formulations.

4 , 4 , 2014

177 - 181