EFFECT OF SUSPENSION CULTURE ON BASIL AND ANISE ESSENTIAL OILS COMPOSITION AND COMPARISON OF THEIR CYTOTOXIC, IMMUNOSTIMULANT AND ANTIOXIDANT ACTIVITIES
B. Ramya Sree
Department of Biotechnology, Sree Vidyanikethan Engineering College, Tirupati
Jyothi M. Joy
Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati
Suspension cultures initiated from leaf explants of Ocimum basilicum and hypocots of Pimpinella anisum showed major changes in their essential oil composition. Eugenol, α-cubebene, methyl cinnamate and iso-caryophyllene completely disappeared, while Estragole, Methyl eugenol and 10-Methylicosane where the major components newly formed in O. basilicum. However, estragole, limonene, carvone, dihydrocarvyl-acetate and β-caryophyllene vanished, while p-anisaldhyde and p-anisaldhyde dimethyl acetal were biosynthesized in a large percentage. Anise oil was more potent than basil oil as antioxidant confirmed by DPPH and was more cytotoxic on Caco-2 and HepG-2 cell lines. Conversely, basil oil possessed more immunostimulant activity than anise oil confirmed by stimulation index on PBMCs
5 , 4 , 2015
295 - 300