Malaria is a serious disease caused by protozoan parasite Plasmodium of human blood, if left untreated, can be fatal. A number of different diagnostic methods are now in use for diagnosis of malaria, but they are expensive and have less specificity and sensitivity. To that using buffy coat technique to increase the specificity and sensitivity of diagnosis than using the stained blood films. Blood samples were collected from Khartoum teaching hospital. A total number of 40 samples were examined by The Giemsa stain and the buffy coat technique. 32 samples were found to be negative by Giemsa stain and Buffy coat and eight (8) samples examined were found to be positive.4 out of 8 samples were found to be positive by microscopy for presence of parasites by Giemsa stain and the same 4 samples were found to be positive by Buffy coat, while 4 of them were positive by Buffy coat only. The sensitivity and specificity of the Buffy coat technique were found to be 100% and88.8% respectively compared to microscopic examination. Conclusion the Buffy coat technique was more sensitive and specific than microscopy, and was simple to apply.