The present study was carried out to evaluate the anti-arthritic activity of ethanolic of extract Tinospora sinensis leaf (EETS) in adjuvant induced arthritis in rats. Loss in body weight during arthritis condition was corrected on treatment with EETS and standard drug, indomethacin. Biochemical parameters such as hemoglobin content, total WBC, RBC, erythrocyte and sedimentation rate were also estimated. The ethanolic extract at the dose of 300 mg/kg body weight inhibited the rat paw edema by 68.32% which is comparable with standard drug indomethacin 75.87% inhibition of rat paw edema after 21 days. The results of the current investigation concluded that EETS possess a significant anti-arthritic activity against adjuvant induced arthritis and justifying its therapeutic role in arthritic condition. The observed antiarthritic activity may be due to the presence of phytoconstituents such as alkaloids, phenolic compounds and flavonoids.