The aim of this study was to formulate and evaluate Acyclovir sodium controlled release matrix tablets. Acyclovir sodium controlled release tablets were prepared by non aqueous wet granulation method using tamarind seed polysaccharide (natural polymer) with six percentages of polymers (10%, 15%, 20%, 25%, 30% and 35%). No interaction was found between drug and polymer through the Fourier transform infrared spectroscopy (FT-IR). The prepared granules were evaluated to preformulation studies such as angle of repose, bulk density, tapped density, bulkiness, and compressibility index and hausner‟s ratio. All the parameters proved that the granules having good flow properties. Then the formulated tablets were taken to evaluation studies such as hardness, weight variation, friability, drug content and thickness. All the parameters were within the acceptable limits. The in vitro release study was performed in phosphate buffer pH 7.4 at 253 nm. The in vitro release study showed that if the polymer ratio is increased, then the retarding efficiency of drug release also increased.