The combination of these two drugs (Timolol maleate and Travoprost) is used to lower the pressure in the eye when one of the medicines does not lower the pressure enough on its own. The eye drops should be layed into the affected eye(s) once per day. The aim of the present study is to develop new analytical method for the simultaneous estimation of Timolol maleate and Travoprost in bulk and in Pharmaceutical dosage form. Spectroscopic method have been developed for the quantification of Timolol maleate and Travoprost in bulk and in the formulation. In simultaneous equation method the absorbance of the Timolol maleate and Travoprost measured at 234nm and 280nm respectively by using double distilled water as blank which was further applied for determining the concentration of the both the drugs in formulation. The proposed method validated statistically for specificity, linearity, accuracy and precision. The LOD value of Timolol maleate and Travoprost was found to be 0.043, 0.0021 respectively. LOQ value of Timolol maleate and Travoprost was found to be 0.142, 0.0069 respectively. This method is validated as per ICH guidelines. This method is simple, cost effective, accurate and precise.